Human Phospho-FAK (Y397) Antibody
Human Phospho-FAK (Y397) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-FAK (Y397) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-FAK (Y397) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4528), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FAK (Y397) at approximately 122 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Phospho-FAK (Y397) by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-FAK (Y397) at approximately 123 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-FAK (Y397) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4528). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Phospho-FAK (Y397) in HUVECs. FAK phosphorylated at Y397 was detected in immersion fixed HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV) using Rabbit Anti-Human Phospho-FAK (Y397) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4528) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclear and focal adhesions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FAK
Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. FAK autophosphorylation at Tyr397 by integrin clustering creates a high affinity binding site recognized by the SH2 domain of Src family kinases and leads to the recruitment and activation of Src. Additionally, other SH2 containing proteins are recruited by the phosphorylation of Tyr397, including Grb7, PLC-gamma, and the 85 kDa subunit of PI3 kinase. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.
Product Datasheets
Citation for Human Phospho-FAK (Y397) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Breast Cancer Malignant Processes are Regulated by Pax-5 Through the Disruption of FAK Signaling Pathways
Authors: S Benzina, J Harquail, R Guerrette, P O'Brien, S Jean, N Crapoulet, GA Robichaud
J Cancer, 2016-10-22;7(14):2035-2044.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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