* Provided that the recommended microplates, buffers, diluents, substrates and solutions
are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human PDGF R alpha in cell lysates. An immobilized capture antibody specific for PDGF R alpha binds both phosphorylated and unphosphorylated PDGF R alpha. After washing away unbound material, an HRP-conjugated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.
Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15-(96-well) plate pack sizes
Economical alternative to Western blot
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF R alpha
PDGF R alpha (Platelet-Derived Growth Factor Receptor alpha) is a transmembrane receptor tyrosine kinase that can form homodimers or heterodimers with PDGF R beta when engaged by dimers of the PDGF ligands. PDGF R alpha binds to PDGF-AA, PDGF-AB, and PDGF-CC. It is strongly expressed in oligodendrocyte, lung, skin, and intestinal progenitor cells, at low levels in most mesenchymal cells, and can be induced by inflammation. PDGF R alpha function is important during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme. Dysregulated PDGF R alpha signaling can result in spina bifida or cleft palate-type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart, and skin (scleroderma).
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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