Human Phospho-VEGF R1/Flt-1 (Y1213) Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human VEGF R1/Flt-1 when phosphorylated at Y1213.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human VEGF R1/Flt-1 Y1213 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-VEGF R1/Flt‑1 (Y1213) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-VEGF R1/Flt‑1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti‑Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGF R1/Flt‑1 (Y1213) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R1/Flt-1

VEGF R1, also known as Flt-1, is a receptor tyrosine kinase that contains seven Ig-like repeats in its extracellular domain, a single transmembrane region, and a kinase domain in the intracellular region. Ligand binding induces autophosphorylation on multiple intracellular tyrosine residues. Y1213 is a major binding site for PI3K, Nck, and SHP-2.

  • Long Name:
    Vascular Endothelial Growth Factor Receptor 1
  • Entrez Gene IDs:
    2321 (Human); 14254 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cells
    Authors: MG Atzori, L Tentori, F Ruffini, C Ceci, L Lisi, E Bonanno, M Scimeca, E Eskilsson, T Daubon, H Miletic, L Ricci Viti, R Pallini, P Navarra, R Bjerkvig, S D'Atri, PM Lacal, G Graziani
    J. Exp. Clin. Cancer Res., 2017;36(1):106.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Deletion of placental growth factor prevents diabetic retinopathy and is associated with Akt activation and HIF1alpha-VEGF pathway inhibition.
    Authors: Huang H, He J, Johnson D, Wei Y, Liu Y, Wang S, Lutty G, Duh E, Semba R
    Diabetes, 2015;64(1):200-12.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  3. BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
    Authors: Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi R, Xu W, Karakousis G, Gangadhar T, Schuchter L, Lieu M, Khare S, Halloran M, Herlyn M, Kaufman R
    Clin Cancer Res, 2015;21(7):1652-64.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells.
    Authors: Attar-Schneider O, Drucker L, Zismanov V
    Lab. Invest., 2012;92(2):178-90.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
    Authors: Meda C, Molla F, De Pizzol M
    J. Immunol., 2012;188(8):4081-92.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
    Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
    J. Biol. Chem., 2008;283(49):34250-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
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Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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