Human PI 3-Kinase p110δ Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human PI 3-kinase p110δ in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 275144
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PI 3-Kinase p110δ
    Cys391-Phe542
    Accession # O00329
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PI 3-Kinase p110δ by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3‑Kinase p110δ Monoclonal Antibody (Catalog # MAB2687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3‑Kinase p110 delta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p110 delta

Class IA phosphoinositide 3-kinases (PI 3-kinases) are a family of p85/p110 heterodimeric lipid kinases that function as signal transducers downstream of growth factor receptors, mainly receptor tyrosine kinases. Mammals express three class IA catalytic subunits: p110 alpha, p110 beta, and p110δ. The p110δ subunit is expressed predominantly in leukocytes, indicating that it may play a role in immune signaling.

  • Long Name:
    Protein Tyrosine Phosphatase 1D
  • Entrez Gene IDs:
    5293 (Human)
  • Alternate Names:
    EC 2.7.1; EC 2.7.1.153; p110D; P110DELTA; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphoinositide-3-kinase C; phosphoinositide-3-kinase, catalytic, delta polypeptide; PI 3Kinase p110 delta; PI 3-Kinase p110 delta; PI3K; PI3K-delta; PI3-kinase p110 subunit delta; PI3-kinase subunit delta; PIK3CD; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. PI3K? and PI3K? isoforms have distinct functions in regulating pro-tumoural signalling in the multiple myeloma microenvironment
    Authors: RE Piddock, N Loughran, CR Marlein, SD Robinson, DR Edwards, S Yu, GE Pillinger, Z Zhou, L Zaitseva, MJ Auger, SA Rushworth, KM Bowles
    Blood Cancer J, 2017;7(3):e539.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Identification of HSP90 inhibitors as a novel class of senolytics
    Authors: H Fuhrmann-S, YY Ling, J Zhao, SJ McGowan, Y Zhu, RW Brooks, D Grassi, SQ Gregg, JL Stripay, A Dorronsoro, L Corbo, P Tang, C Bukata, N Ring, M Giacca, X Li, T Tchkonia, JL Kirkland, LJ Niedernhof, PD Robbins
    Nat Commun, 2017;8(1):422.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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