Detection of Human PKCδ by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line and HT‑29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PKCδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4585) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKCδ at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC delta
PKCδ (protein kinase C-delta) is a 78 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++‑independent and phospholipid‑dependent ubiquitous enzyme that participates in cellular apoptosis. Human PKCδ is 676 amino acids (aa) in length. It contains two general regions: a phosphotyrosine and DAG-type 2 binding region (aa's 1‑90 and 159‑208, respectively), and an ATP-binding catalytic region (aa 349‑603). Functionally, inactive cytoplasmic PKCδ is first activated by Tyr64 and Tyr155 phosphorylation. This induces PKCδ entry into the nucleus, where it is cleaved into 43 kDa and 36 kDa fragments, initiating apoptosis. There are two potential alternate start sites for PKCδ at Met51 and Met74. In some cells, a poorly characterized 160 kDa form of PKCδ has been noted. Over aa 549‑676, human PKCδ is 85% aa identical to mouse PKCδ.
Protein Kinase C delta
Entrez Gene IDs:
EC 2.7.11; EC 188.8.131.52; MAY1; MGC49908; nPKC-delta; PKC delta; PKCD; PRKCD; protein kinase C delta type; protein kinase C delta VIII; protein kinase C, delta
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