Human Plexin D1 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Plexin D1 in ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 752829
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Plexin D1
    Accession # Q9Y4D7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Plexin D1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Plexin D1 PE‑conjugated Monoclonal Antibody (Catalog # FAB7536P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Plexin D1

Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors (1, 2). Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins (1). The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and ~84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron (1, 2). Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus (3). Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct (4). Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities (4, 5). Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development (2, 6). In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal (7, 8). Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E (9). Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands (10, 11).

  • References:
    1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
    2. Van Der Zwaag, B. et al. (2002) Dev. Dyn. 225:336.
    3. Entrez protein Accession # Q9Y4D7, EAW79239, EAW79240.
    4. Gu, C. et al. (2005) Science 307:265.
    5. Gitler, A.D. et al. (2004) Developmental Cell 7:107.
    6. Zhang, Y. et al. (2009) Dev. Biol. 325:82.
    7. Chauvet, S. et al. (2007) Neuron 56:807.
    8. Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
    9. Choi, Y.I. et al. (2008) Immunity 29:888.
    10. Toyofuku, T. et al. (2007) EMBO J. 26:1373.
    11. Roodink, I. et al. (2008) Am. J. Pathol. 173:1873.
  • Entrez Gene IDs:
    23129 (Human); 67784 (Mouse)
  • Alternate Names:
    KIAA0620MGC75353; PLEXD1; plexin D1; plexin-D1; PLXND1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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