Human PLZF Antibody Summary
Accession # Q05516
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PLZF by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human PLZF Monoclonal Antibody (Catalog # MAB2944) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PLZF in HL‑60 Human Cell Line. PLZF was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Human PLZF Monoclonal Antibody (Catalog # MAB2944) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of in HL‑60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Human PLZF Monoclonal Antibody (Catalog # MAB2944, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.
Citations for Human PLZF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Detrimental effects of flame retardant, PBB153, exposure on sperm and future generations
Authors: KW Greeson, KL Fowler, PM Estave, S Kate Thomp, C Wagner, R Clayton Ed, KM Symosko, AN Steves, EM Marder, ML Terrell, H Barton, M Koval, M Marcus, CA Easley
Sci Rep, 2020;10(1):8567.
Sample Types: Whole Cells
PLZF, a tumor suppressor genetically lost in metastatic castration-resistant prostate cancer, is a mediator of resistance to androgen deprivation therapy.
Authors: Hsieh C, Botta G, Gao S, Li T, Van Allen E, Treacy D, Cai C, He H, Sweeney C, Brown M, Balk S, Nelson P, Garraway L, Kantoff P
Cancer Res, 2015;75(10):1944-8.
Sample Types: Cell Lysates
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