Human PLZF Antibody

AF2944 11 Citations Datasheet / COA / SDS

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AF2944
AF2944-SP

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Citations (11)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PLZF Antibody Summary

Species Reactivity

Human

Specificity

Detects human PLZF in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PLZF
Met1-Gln254
Accession # Q05516

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human PLZF by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human PLZF by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLZF

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Long Name

Promyelocytic Leukemia Zinc Finger

Entrez Gene IDs

7704 (Human); 235320 (Mouse)

Alternate Names

PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PLZF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
Authors: RL Tang, LQ Fan
Asian J. Androl., 2019;21(3):309-318.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Biological and RNA regulatory function of MOV10 in mammalian germ cells
Authors: K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng
BMC Biol., 2019;17(1):39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-F
EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis
Authors: SP Jamin, FG Petit, C Kervarrec, F Smagulova, D Illner, H Scherthan, M Primig
Sci Rep, 2017;7(1):15065.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis
Authors: K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig
Biol. Reprod., 2017;96(3):707-719.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P
The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells
Nat. Immunol, 2016;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Multiple layers of transcriptional regulation by PLZF in NKT-cell development
Authors: Ai-Ping Mao
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.
Authors: Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J
PLoS ONE, 2015;10(11):e0143148.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
PAX7 expression defines germline stem cells in the adult testis.
Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
J Clin Invest, 2014;124(9):3929-44.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture.
Authors: Makela J, Toppari J, Rivero-Muller A, Ventela S
PLoS ONE, 2014;9(3):e90088.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
PLZF mediates the PTEN/AKT/FOXO3a signaling in suppression of prostate tumorigenesis.
Authors: Cao, JingPing, Zhu, Shu, Zhou, Wei, Li, Jie, Liu, Chang, Xuan, HanQing, Yan, Jie, Zheng, Lin, Zhou, LiXin, Yu, JianXiu, Chen, GuoQiang, Huang, YiRan, Yu, Zhuo, Feng, LiXin
PLoS ONE, 2013;8(12):e77922.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
Authors: Mathew, Rebecca, Seiler, Michael, Scanlon, Seth T, Mao, Ai-ping, Constantinides, Michael, Bertozzi-Villa, Clara, Singer, Jeffrey, Bendelac, Albert
Nature, 2012;491(7425):618-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot