Detection of PLZF in HL‑60 Human Cell Line by Flow Cytometry.
HL‑60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human PLZF PerCP‑conjugated Monoclonal Antibody (Catalog # IC2944C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.
Promyelocytic Leukemia Zinc Finger
Entrez Gene IDs:
7704 (Human); 235320 (Mouse)
PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein
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