Human PPAR gamma /NR1C3 Antibody

Catalog # Availability Size / Price Qty
PP-A3409A-00
Product Details
Citations (1)
FAQs
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Human PPAR gamma /NR1C3 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human PPAR gamma 1 and gamma 2 and cross-reacts with mouse and rat PPAR gamma 1 and gamma 2. This antibody does not recognize human PPAR alpha and PPARδ. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # A3409A
Purification
Protein A or G purified from ascites
Immunogen
Recombinant human PPAR gamma/NR1C3
Amino Acids 3-108
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human PPAR gamma.
 
Immunohistochemistry
This antibody can be used at 10 μg/mL with the appropriate secondary reagents to detect human PPAR gamma.
 
Immunoprecipitation
Optimal dilutions should be determined by each laboratory.
 
Gel Supershift Assay
Optimal dilutions should be determined by each laboratory.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PPAR gamma/NR1C3

Peroxisome Proliferator-Activated Receptor gamma (PPAR gamma ; NR1C3) is a member of the orphan nuclear receptor family. Oxidized metabolites of linoleic acid, 9-hydroxyctadienoic acid (9-HODE) and 13-HODE are activators and ligands of PPAR gamma. PPAR gamma is expressed in white adipose tissue, intestinal mucosa, colon, spleen, monocytes, macrophages, retina, cartilage, osteoclasts, and skeletal muscle. PPAR gamma plays important roles in lipid and glucose metabolism and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three known family members are called PPAR alpha, δ, and gamma. Three N-terminal isoforms, called gamma 1, gamma 2 and gamma 3, are known to arise by alternative splicing and promoter usage from the PPAR gamma gene. RXR is an obligate partner for PPAR.

Long Name
Peroxisome Proliferator-activated Receptor gamma
Entrez Gene IDs
5468 (Human)
Alternate Names
CIMT1; NR1C3; NR1C3GLM1; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma; PPAR gamma; PPARG; PPARG1peroxisome proliferative activated receptor, gamma; PPARG2PPARgamma; PPAR-gamma

Product Datasheets

Citation for Human PPAR gamma /NR1C3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mutant p53 controls tumor metabolism and metastasis by regulating PGC-1?
    Authors: S Basu, K Gnanaprade, T Barnoud, CP Kung, M Tavecchio, J Scott, A Watters, Q Chen, AV Kossenkov, ME Murphy
    Genes Dev., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay (PLA)

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

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