Human PRDM14 Antibody Summary
Accession # Q9GZV8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PRDM14 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells (undifferentiated) and NTera‑2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human PRDM14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6175) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM14 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PRDM14 in BG01V Human Embryonic Stem Cells. PRDM14 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human PRDM14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6175) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel; Catalog # NL012) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400‑568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up‑regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.
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