Human/Primate IL-17F Antibody

Catalog # Availability Size / Price Qty
AF1335
AF1335-SP
Human/Primate IL-17F Antibody in Data
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Primate IL-17F Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human IL-17F in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity recombinant human (rh) IL‑17, rhIL‑17B, rhIL-17C, rhIL-17D, rhIL-17E, and recombinant mouse IL-17F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-17F (R&D Systems, Catalog # 1335-IL)
Arg31-Gln163
Accession # AAK83350
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑17F (Catalog # 1335-IL)
Immunohistochemistry
3-15 µg/mL
See below
Dual RNAscope ISH-IHC
3-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells treated with PMA and ionomycin.

 

Human/Primate IL-17F Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate IL‑17F Biotinylated Antibody (Catalog # BAF1335)

Standard: Recombinant Human IL-17F Protein (Catalog # 1335-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry IL-17F antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑17F in Human Tonsil. IL-17F was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human/Primate IL-17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1335) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Dual RNAscope ISH-IHC Compatible IL-17F antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

IL-17F in Human Tonsil Using Dual RNAscope®ISH and IHC. IL-17F mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe (Catalog # 310941) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL-17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1335) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17F cDNA encodes a 163 aa protein with a putative 30 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17A (approximately 44% aa sequence homology), but shares only limited sequence homology (16 - 30%) with IL-17B, C, D and E. Human and mouse IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T-cells and activated monocytes. Five receptors (IL-17 RA, B, C, D and E) have been identified (5). Although the ligands for IL-17 RD and E are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL-17A and IL-17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (5, 6). The biological activities mediated by IL-17F are similar to those of IL‑17. IL‑17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL‑17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (3).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  4. Shen, F. & S. L. Gaffen (2008) Cytokine 41:92.
  5. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

Product Datasheets

Citations for Human/Primate IL-17F Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
    Authors: Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D
    Respir Res, 2014;15(1):145.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
    Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
    Chest, 2011;138(5):1140-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Tripeptidyl-peptidase II controls DNA damage responses and in vivo gamma-irradiation resistance of tumors.
    Authors: Hong X, Lei L, Kunert B, Naredla R, Applequist SE, Grandien A, Glas R
    Cancer Res., 2007;67(15):7165-74.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Primate IL-17F Antibody
By Anonymous on 01/09/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human