Detection of Human PSG-6 by Western Blot.
Western blot shows human serum from a pregnant donor (+) compared to a non-pregnant donor (-). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PSG-6 Monoclonal Antibody (Catalog # MAB8598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PSG-6 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PSG-6 in Human Placenta.
PSG-6 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human PSG-6 Monoclonal Antibody (Catalog # MAB8598) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Pregnancy-specific beta-1-glycoprotein 6 is encoded by the PSG6 gene. High levels of PSG biosynthesis are restricted to the placenta
syncytiotrophoblast and are essential for the maintenance of normal
gestation in mammalian species.
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