Human/Rat IL-32 beta Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB6769-100
MAB6769-SP
Human/Rat IL-32 beta  Antibody in Western Blot
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Human/Rat IL-32 beta Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human IL‑32 beta in direct ELISAs. Detects human and rat IL‑32 beta in direct Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2525B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived human IL‑32 beta protein
Met1-Lys188
Accession # NP_001012649
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human and Rat IL‑32 beta  by Western Blot. View Larger

Detection of Human and Rat IL‑32 beta by Western Blot. Western blot shows lysates of Nb2‑11 rat lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Rat IL‑32 beta Monoclonal Antibody (Catalog # MAB6769) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IL‑32 beta at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-32  beta in Human PBMCs. View Larger

IL-32 beta in Human PBMCs. IL‑32 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated or untreated with calcium ionomycin and PMA using Rabbit Anti-Human/Rat IL‑32 beta Monoclonal Antibody (Catalog # MAB6769) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-32 beta

Interleukin 32 (IL‑32) is an N‑glycosylated cytokine that is up-regulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and vascular endothelial cells as well as in activated T cells (1‑6). It cooperates with inflammatory stimuli to promote the expression of other proinflammatory molecules such as TNF‑ alpha, IL‑6, IL‑1 beta, IL‑1 alpha, and CXCL8/IL‑8 (5‑9). The longest of several IL‑32 splicing variants is the 234 amino acid gamma isoform which is also known as natural killer cell transcript 4 (NK4) (9). The 25 kDa beta isoform (IL‑32 beta ) lacks aa 19‑64 including a portion of the putative signal peptide. Neutrophil‑derived Proteinase 3 (PR3) cleaves IL‑32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL‑32 isoforms (10). The alpha, beta, gamma, delta, epsilon, and zeta isoforms show different degrees of antiviral activity against influenza virus replication with IL‑32 gamma being the most potent (11). IL‑32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (6, 12‑14). IL‑32 inhibits HIV‑1 replication in vitro, and it is elevated in the serum of HIV‑1 patients (15, 16).

References
  1. Netea, M.G. et al. (2006) PloS Med. 3:e277.
  2. Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. 106:3883.
  3. Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
  4. Goda, C. et al. (2006) Int. Immunol. 18:233.
  5. Choi, J.-D. et al. (2009) Immunology 126:535.
  6. Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
  7. Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. 102:16309.
  8. Hong, J. et al. (2010) Cytokine 49:171.
  9. Kim, S.-H. et al. (2005) Immunity 22:131.
  10. Novick, D. et al. (2006) Proc. Natl. Acad. Sci. 103:3316.
  11. Li, W. et al. (2010) J. Immunol. 185:5056.
  12. Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
  13. Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. 103:3298.
  14. Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
  15. Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
  16. Nold, M.F. et al. (2008) J. Immunol. 181:557.
Long Name
Interleukin 32 beta
Alternate Names
IL32 beta; IL-32 beta; IL32; IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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