Human/Rat MAD2L1 Antibody

Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human/Rat MAD2L1 by Western Blot.
2 Images
Product Details
Supplemental Products

Human/Rat MAD2L1 Antibody Summary

Species Reactivity
Human, Rat
Detects human and rat MAD2L1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human MAD2L1
Accession # Q13257
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat MAD2L1 antibody by Western Blot. View Larger

Detection of Human/Rat MAD2L1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and NRK rat normal kidney cells. PVDF membrane was probed with 1 µg/mL of Human/Rat MAD2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MAD2L1 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Immunocytochemistry MAD2L1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

MAD2L1 in A549 Human Cell Line. MAD2L1 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Rat MAD2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4005) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAD2L1

MAD2L1 (Mitotic arrest deficient protein 2) is a component of the spindle-attachment checkpoint mechanism that monitors kinetochore spindle attachment and leads to the subsequent arrest in early metaphase by its recruitment to unattached kinetochores. The transition from metaphase to anaphase requires the association of the anaphase promoting complex/cyclosome (APC/C) with Cdc20 leading to the ubiquitylation and subsequent degradation of Pds1/Securin. This transition is delayed by the inhibitory association of MAD2L1 with Cdc20. MAD2L1 has also been shown to be a direct E2F target and as such is aberrantly expressed in cells with retinoblastoma pathway defects.

Long Name
MAD2 Mitotic Arrest Deficient-Like 1
Entrez Gene IDs
4085 (Human); 56150 (Mouse)
Alternate Names
HSMAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2L1; MAD2-like protein 1; MAD2mitotic arrest deficient, yeast, homolog-like 1; Mitotic arrest deficient 2-like protein 1; mitotic spindle assembly checkpoint protein MAD2A

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Rat MAD2L1 Antibody

There are currently no reviews for this product. Be the first to review Human/Rat MAD2L1 Antibody and earn rewards!

Have you used Human/Rat MAD2L1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review