PKC beta 1 in Rat Cortical Stem Cells. PKC beta 1 was detected in immersion fixed rat cortical stem cells using Goat Anti-Human/Rat PKC beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC beta 1
Protein Kinase C (PKC) beta 1 is a member of the PKC family of serine/threonine protein kinases. It is 671 amino acids (aa) in length with a predicted molecular mass of 77 kDa. Human PKC beta 1 shares 98% aa sequence identity with the mouse and rat orthologs. A second PKC beta splice variant, PKC beta 2, contains an alternate C-terminal sequence. Conventional PKC isoforms, including PKC beta 1, require calcium and phosphatidylserine, diacylglycerol (DAG), or exogenous phorbol esters for activation. PKC beta 1 plays a role in B cell receptor signaling and histone demethylation.
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