Human SCARA5 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4900G-100UG
Human SCARA5 Alexa Fluor® 488-conjugated Antibody in Data
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Human SCARA5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SCARA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SCARA5 is observed.
Source
Monoclonal Mouse IgG2B Clone # 673527
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human SCARA5
Arg83-His495
Accession # Q6ZMJ2
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SCARA5 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human SCARA5 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4900G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SCARA5

Scavenger Receptor A5 (SCARA5), also known as Tesr, is a 70-75 kDa kDa member of the scavenger receptor "supergroup" of molecules. It is one of at least six class A members that possess a collagen-like domain and form intramembrane homotrimers (1,3). Class A scavenger receptors are type II transmembrane proteins. Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a Scavenger Receptor Cysteine-Rich (SRCR) domain (3-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. Among other class A members, it shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1 in the ECD. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of 200-210 kDa (5,7). It is highly expressed by a number of cell types, including testicular Sertoli cells, transitional epithelium, respiratory pseudostratified columnar epithelium, intestinal M cells, astrocytes, retinal ganglion cells, endothelial cells and likely intestinal Paneth cells (5, 8). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5). It is also recognized to bind and transport L (light)-ferritin across anatomical boundaries (8). Finally, SCARA5 has also been found in both nucleus and cytoplasm (8).

References
  1. Martinez, V.G. et al. (2011) Pharmacol. Rev. 63:967.
  2. Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
  3. Zani, I.A. et al. (2015) Cells 4:178.
  4. Sarraj, M.A. et al. (2005) Dev. Dyn. 234:1026.
  5. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
  6. SwissProt Accession # Q6ZMJ2.
  7. Li, J.Y. et al. (2009) Dev. Cell 16:35.
  8. Mendes-Jorge, L. et al. (2014) PLoS One. 9:e106974.
Long Name
Scavenger Receptor Class A Member 5
Entrez Gene IDs
286133 (Human); 71145 (Mouse); 305974 (Rat)
Alternate Names
FLJ23907; MGC45780; SCARA5; scavenger receptor class A, member 5 (putative); Tesr; testis expressed scavenger receptor; UNQ2938/PRO28700

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