Detects human Semaphorin 4A in direct ELISAs. In direct ELISAs, 50%-100% cross-reactivity with
recombinant human (rh) Semaphorin 4C and rhSemaphorin 4G is observed, and less
than 5% cross-reactivity with rhSemaphorin 4B, 4D, and recombinant mouse
Semaphorin 4A is observed.
Monoclonal Mouse IgG1 Clone # 741531
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A Gly32-His683 Accession # Q9H3S1
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Semaphorin 4A in Human PBMC Lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell lymphocytes, unactivated (panel A) or activated with PMA and calcium ionomycin (panel B), were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and Mouse Anti-Human Semaphorin 4A APC‑conjugated Monoclonal Antibody (Catalog # FAB4694A). Quadrant gates are set based on staining with Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4A
Semaphorin 4A (Sema4A), previously SemaB, is a 145-150 kDa Class IV transmembrane Semaphorin with activity in the immune and nervous systems (1). The 761 amino acid (aa) human Sema4A precursor contains a 32 aa signal sequence, a 651 aa extracellular domain (ECD) containing sema, PSI and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 57 aa cytoplasmic domain with two Ser/Thr phosphorylation sites (2). Human Sema4A ECD shares 87%, 87%, 86% and 85% aa identity with mouse, rat, bovine and canine Sema4A, respectively, and shares 32-37% aa identity with other human Sema4 family members. Of six reported splice variants, 723, 629, 370, 321, 236 and 220 aa, five lack the N-terminus and/or portions of the sema domain, and three lack the transmembrane and cytoplasmic domains in the C-terminus (3). Sema4A was first described as a molecule that enhances T cell activation and interacts with TIM-2 (T cell immunoglobulin and mucin domain-2) on Th2 cells (4). Mice with targeted disruption of Sema4A show defects in dendritic cell-mediated T cell priming and Th1 responses (6). Roles for Sema4A have also been identified in the brain, the vasculature, and the eye. It mediates hippocampal neuron growth cone collapse in vitro through interaction of the sema domain with Plexin-B1 (7). Interaction of Sema4A with macrophage Plexin D1 promotes VEGF production and an angiogenic phenotype (8). Finally, the retina of Sema4A-/- mice shows severe degeneration, and mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans (8, 9). This may be due to a malfunction of endosomal sorting mediated by Sema4A (9,10).
Nkyinbeng-Takwi, E. and S.P. Chapoval (2011) Immunol. Res 50:10.
Swissprot Accession # Q9H3S1.
Entrez Accession # CAI15528, CAI15529, CAI15531, CAI15532, CAI15533 and EAW52993.
Kumanogoh, A. et al. (2002) Nature 419:629.
Yukawa, K. et al. (2010) Int. J. Mol. Med. 25:225.
Kumanogoh, A. et al. (2005) Immunity 22:305.
Yukawa, K. et al. (2005) Int. J. Mol. Med. 16:115.
Meda, C. et al. (2012) J. Immunol. 188:4081.
Rice, D.S. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:2767.
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