Human Semaphorin 4B PE-conjugated Antibody

FAB5485P has been discontinued. View all Semaphorin 4B products.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Semaphorin 4B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Semaphorin 4B is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 561416
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4B
    Leu39-Glu712
    Accession # Q9NPR2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Semaphorin 4B in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Semaphorin 4B PE‑conjugated Monoclonal Antibody (Catalog # FAB5485P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4B
Semaphorin 4B (Sema4B), also known as SemaC, is a 95‑100 kDa, class IV member of the Semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids (aa) in length. It contains a 674 aa extracellular region (aa 39‑832) that is characterized by one Sema domain (aa 65‑502), a PSI region (aa 520‑574), and a C2-type Ig-like domain (aa 599‑658). There is one potential soluble splice variant that shows a 29 aa substitution for aa 707‑832. Over aa 39‑712, human Sema4B shares 86% aa sequence identity with mouse Sema4B.
  • Entrez Gene IDs:
    10509 (Human); 20352 (Mouse); 293042 (Rat)
  • Alternate Names:
    KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B; MGC131831; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B; SEMA4B; SEMAC; Semaphorin 4B; semaphorin-4B; SemC
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