|Detection of Human SGK by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line non-transfected and transfected with human SGK1. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for SGK was detected at approximately 54 kDa (as indicated). For additional reference, recombinant human SGK1, SGK2, and SGK3 were included. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Serum- and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases. In addition to serum and glucocorticoids, insulin, IGF-I, osmotic shock, and mineralocorticoids have been demonstrated to activate SGK1. Expressed at low levels, SGK1 appears to function as a regulator of epithelial ion transport. Sustained high levels of SGK1 activation have been implicated in hypertension and diabetic nephropathy.
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