Human Siglec-5/Siglec-14 APC-conjugated Antibody

FAB10721A has been discontinued. View all Siglec-5/Siglec-14 products.
(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Siglec‑5 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 194128
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Siglec‑5
    Lys18-Thr434
    Accession # O15389
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Siglec‑5/Siglec‑14 in Human Monocytes by Flow Cytometry. Human monocytes were stained with Mouse Anti-Human Siglec‑5/Siglec‑14 APC‑conjugated Monoclonal Antibody (Catalog # FAB10721A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-5/Siglec-14

Siglecs (Sialic acid binding Ig-like Lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5, also known as CD170, binds to alpha-2,3- and alpha-2,6-linked sialic acid with similar affinity. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in monocytes and neutrophils. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.

  • Long Name:
    Sialic Acid Binding Ig-like Lectin 5
  • Entrez Gene IDs:
    8778 (Human)
  • Alternate Names:
    Siglec-5/Siglec-14
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy
    Sci Rep, 2016;6(0):36012.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
    Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
    Immunol. Lett., 2011;134(2):113-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Relative over-reactivity of human versus chimpanzee lymphocytes: implications for the human diseases associated with immune activation.
    Authors: Soto PC, Stein LL, Hurtado-Ziola N, Hedrick SM, Varki A
    J. Immunol., 2010;184(8):4185-95.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Type: Whole Cells
    Application: Flow

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