Detects human Siglec‑5 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Monoclonal Mouse IgG1 Clone # 194128
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5 Lys18-Thr434 Accession # O15389
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Siglec‑5/Siglec‑14 in Human Monocytes by Flow Cytometry. Human monocytes were stained with Mouse Anti-Human Siglec‑5/Siglec‑14 APC‑conjugated Monoclonal Antibody (Catalog # FAB10721A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Siglecs (Sialic acid binding Ig-like Lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5, also known as CD170, binds to alpha-2,3- and alpha-2,6-linked sialic acid with similar affinity. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in monocytes and neutrophils. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.
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