Human Siglec-5/Siglec-14 Fluorescein-conjugated Antibody

Discontinued Product

FAB10721F has been discontinued.
Detection of Siglec‑5/Siglec‑14 in Human Monocytes by Flow Cytometry.
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Product Details
Citations (2)
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Human Siglec-5/Siglec-14 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec‑5 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 194128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5
Lys18-Thr434
Accession # O15389
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Siglec‑5/Siglec‑14 in Human Monocytes by Flow Cytometry. View Larger

Detection of Siglec‑5/Siglec‑14 in Human Monocytes by Flow Cytometry. Human monocytes were stained with Mouse Anti-Human Siglec‑5/Siglec‑14 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB10721F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-5/Siglec-14

Siglecs (Sialic acid binding Ig-like Lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5, also known as CD170, binds to alpha-2,3- and alpha-2,6-linked sialic acid with similar affinity. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in monocytes and neutrophils. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.

Long Name
Sialic Acid Binding Ig-like Lectin 5
Entrez Gene IDs
8778 (Human)
Alternate Names
Siglec-5/Siglec-14

Product Datasheets

Citations for Human Siglec-5/Siglec-14 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Developing aptamer probes for acute myelogenous leukemia detection and surface protein biomarker discovery.
    Authors: Yang, Mingli, Jiang, Guohua, Li, Wenjing, Qiu, Kai, Zhang, Min, Carter, Christop, Al-Quran, Samer Z, Li, Ying
    J Hematol Oncol, 2014;7(0):5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus.
    Authors: Ali S, Fong J, Carlin A, Busch T, Linden R, Angata T, Areschoug T, Parast M, Varki N, Murray J, Nizet V, Varki A
    J Exp Med, 2014;211(6):1231-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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