Detection of Somatostatin R3/SSTR3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human Somatostatin R3/SSTR3 APC‑conjugated Monoclonal Antibody (Catalog # FAB7018A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Somatostatin R3/SSTR3
Somatostatin Receptor 3 (SSTR3) is one of five related receptors that belong to GPCR family 1. It is a 7-transmembrane glycoprotein whose MW in SDS-PAGE varies from 50-80 kDa (predicted MW is 45 kDa). All five somatostatin receptors couple to Gi-proteins and inhibit adenylyl cyclase. SSTR3 binds both the 14 amino acid (aa) and 28 aa forms of Somatostatin, and in contrast to rat SSTR3, appears to prefer the shorter, or 14 aa form of Somatostatin. SSTR3 mediates a number of effects, including promoting tight junction formation between epithelium, blocking the release of growth hormone in the anterior pituitary, increasing food intake, and blocking cell proliferation. SSTR3 exists as either a homodimer, or as a heterodimer complexed to SSTR2a and MCHR1. Cells known to express SSTR3, either in the cell membrane or internally include gastric ghrelin cells, the cilium associated with multiple CNS neurons and pancreatic beta -cells, somatotrophic acidophils of the anterior pituitary, renal proximal tubule cells, hepatic stellate (or Ito) cells, keratinocytes, mucosal mast cells, multiple tumor cell types, and the longitudinal visceral smooth muscle of the small intestine. In its extracellular domains, human SSTR3 shares 81% aa sequence identity with rat SSTR3, and 28%, 31%, 35% and 36% aa sequence identity with the extracellular domains of human SSTR1, 2, 4 and 5, respectively.
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