Human SOX9 Antibody

Catalog # Availability Size / Price Qty
AF3075
AF3075-SP
Detection  of Human SOX9 by Western Blot.
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (3)

Human SOX9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) SOX10 is observed, and less than 15% rhSOX11, rhSOX12, and rhSOX15 is observed, and less than 5% cross-reactivity with rhSOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX8, rhSOX13, rhSOX17, rhSOX18, rhSOX21, and rhSOX30 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human SOX9 by Western Blot. View Larger

Detection of Human SOX9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SOX9 in HEK293 Human Cell Line. View Larger

SOX9 in HEK293 Human Cell Line. SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry SOX9 in BG01V Human Embryonic Stem Cells. View Larger

SOX9 in BG01V Human Embryonic Stem Cells. SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western Detection  of Human SOX9 by Simple WesternTM. View Larger

Detection of Human SOX9 by Simple WesternTM. Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX9

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Long Name
Transcription Factor SOX9
Entrez Gene IDs
6662 (Human)
Alternate Names
campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SOX9; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9

Product Datasheets

Citations for Human SOX9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
    Authors: SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy
    Nat. Cell Biol., 2019;21(4):430-441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Meis1 coordinates cerebellar granule cell development by regulating Pax6 transcription, BMP signaling and Atoh1 degradation
    Authors: T Owa, S Taya, S Miyashita, M Yamashita, T Adachi, K Yamada, M Yokoyama, S Aida, T Nishioka, YU Inoue, R Goitsuka, T Nakamura, T Inoue, K Kaibuchi, M Hoshino
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes
    Authors: JR Jones, L Kong, MG Hanna, B Hoffman, R Krencik, R Bradley, T Hagemann, J Choi, M Doers, M Dubovis, MA Sherafat, A Bhattachar, C Kendziorsk, A Audhya, A Messing, SC Zhang
    Cell Rep, 2018;25(4):947-958.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion
    Authors: MJ Castle, Y Cheng, A Asokan, MH Tuszynski
    Sci Adv, 2018;4(11):eaau9859.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling
    Authors: Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
  6. Complex bile duct network formation within liver decellularized extracellular matrix hydrogels
    Authors: PL Lewis, J Su, M Yan, F Meng, SS Glaser, GD Alpini, RM Green, B Sosa-Pined, RN Shah
    Sci Rep, 2018;8(1):12220.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
    Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
    Stem Cell Res Ther, 2018;9(1):15.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Restorative effects of human neural stem cell grafts on the primate spinal cord
    Authors: ES Rosenzweig, JH Brock, P Lu, H Kumamaru, EA Salegio, K Kadoya, JL Weber, JJ Liang, R Moseanko, S Hawbecker, JR Huie, LA Havton, YS Nout-Lomas, AR Ferguson, MS Beattie, JC Bresnahan, MH Tuszynski
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells
    Authors: M Zhang, J Ngo, F Pirozzi, YP Sun, A Wynshaw-Bo
    Stem Cell Res Ther, 2018;9(1):67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors
    Authors: II Aigha, B Memon, AK Elsayed, EM Abdelalim
    Stem Cell Res Ther, 2018;9(1):83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus
    Authors: JM Rosin, DM Kurrasch
    J Neuroinflammation, 2018;15(1):181.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preB�tzinger complex
    Authors: A Kottick, CA Martin, CA Del Negro
    Physiol Rep, 2017;5(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos
    Authors: WJ Lee, S Chatterjee, SP Yap, SL Lim, X Xing, P Kraus, W Sun, X Hu, V Sivakamasu, HY Chan, PR Kolatkar, S Prabhakar, T Lufkin
    Biomed Res Int, 2017;2017(0):8932583.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP
  14. SOX9 is an astrocyte-specific nuclear marker in the adult brain outside the neurogenic regions
    Authors: W Sun, A Cornwell, J Li, S Peng, MJ Osorio, NA Su Wanga, A Benraiss, N Lou, SA Goldman, M Nedergaard
    J. Neurosci, 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis
    Authors: C Lin, E Yao, K Zhang, X Jiang, S Croll, K Thompson-P, PT Chuang
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate
    Authors: Emma L Rawlins
    Development, 2016;143(20):3686-3699.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. The development and plasticity of alveolar type 1 cells
    Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
    Development, 2016;143(1):54-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
    Authors: Zhang X, Westerhoff M, Hart J
    Mod Pathol, 2015;28(5):654-61.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
    Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
    Stem Cell Reports, 2015;4(3):419-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  20. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
    Authors: Mahoney J, Mori M, Szymaniak A, Varelas X, Cardoso W
    Dev Cell, 2014;30(2):137-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Two nested developmental waves demarcate a compartment boundary in the mouse lung.
    Authors: Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao
    Nat Commun, 2014;5(0):3923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.
    Authors: Chatterjee S, Sivakamasundari V, Yap S, Kraus P, Kumar V, Xing X, Lim S, Sng J, Prabhakar S, Lufkin T
    BMC Genomics, 2014;15(0):1072.
    Species: Mouse
    Sample Types: Chromatin
    Applications: ChIP
  24. Identification of SOX3 as an XX male sex reversal gene in mice and humans.
    Authors: Sutton E, Hughes J, White S
    J. Clin. Invest., 2011;121(1):328-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  25. The vanishing testis: a histomorphologic and clinical assessment.
    Authors: Antic T, Hyjek EM, Taxy JB
    Am. J. Clin. Pathol., 2011;136(6):872-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010;93(1):86-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines.
    Authors: Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B
    Oncogene, 2009;28(35):3121-31.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD).
    Authors: Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, Wolffenbuttel KP, Drop SL, Veitia RA, Fellous M, Jaubert F, Looijenga LH
    J. Pathol., 2008;215(1):31-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
    Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
    Stem Cells, 2008;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human SOX9 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human SOX9 Antibody
By Anonymous on 03/20/2019
Application: PFA fixed, sectioned, PBS washed, treated for 5 min in cold Methanol. Worked great! Sample Tested: Embryonic lung Species: Mouse

Human SOX9 Antibody
By Anonymous on 09/27/2017
Application: ICC/IF Sample Tested: iPSC differentiated epithelial cells Species: Human

Human SOX9 Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: stem cells Species: Human

For immunohistochemistry & immunocytochemistry application.