This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human SPARC-like 1 / SPARCL1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SPARC-like 1/SPARCL1
SPARC-L1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as Hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins. SPARC-L1 is a widely expressed protein with antiadhesive properties. It is thought to terminate neuronal migration by antagonizing adhesion, and promote extravasation in tonsil high endothelial venules by inducing endothelial cell dissociation. SPARC-L1 binds collagen and contributes to formation of dermal collagen fibrils.
Entrez Gene IDs:
8404 (Human); 13602 (Mouse)
Hevin; High endothelial venule protein; MAST 9; MAST9; PIG33; proliferation-inducing protein 33; SC1; SPARC like 1; SPARCL1; SPARC-like 1 (hevin); SPARC-like 1 (mast9, hevin); SPARC-like 1; SPARC-like protein 1
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