Human ST2/IL-1 R4 APC-conjugated Antibody

(1 Review)
  • Species Reactivity
  • Specificity
    Detects human ST2/IL‑1 R4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-18 RI, and rhST-7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL‑1 R4
    Accession # BAA02233
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of ST2/IL‑1 R4 in KG‑1a Human Cell Line by Flow Cytometry.
KG‑1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL‑1 R4 APC‑conjugated Polyclonal Antibody (Catalog # FAB5231A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ST2/IL-33 R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 Rrp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33’s ability to signal through transmembrane ST2 (10, 13‑15).

  • References:
    1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
    2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
    3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
    4. Li, H. et al. (2000) Genomics 67:284.
    5. Schmitz, J. et al. (2005) Immunity 23:479.
    6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
    7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
    8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
    9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
    10. Palmer, G. et al. (2008) Cytokine 42:358.
    11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
    12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
    13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
    14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
    15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
  • Long Name:
    Interleukin 33 Receptor
  • Entrez Gene IDs:
    9173 (Human); 17082 (Mouse)
  • Alternate Names:
    DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2L; ST2V; suppression of tumorigenicity 2; T1
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Isotype Controls
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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 Flow Human Anonymous 05/17/2016


ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)

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