Human STAT6 (Y641) PE-conjugated Antibody

Recombinant Monoclonal Antibody
  
Product Details
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human STAT6 when phosphorylated at Y641.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1248D
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing human STAT6 Y641 site.
    Accession # P42226
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of STAT6 in Human peripheral blood mononuclear cells (PBMCs) by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or
(B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) PE‑conjugated Monoclonal Antibody (Catalog # IC37173P) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT6
Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.
  • Long Name:
    Signal Transducer and Activator of Transcription 6
  • Entrez Gene IDs:
    6778 (Human); 20852 (Mouse); 362896 (Rat)
  • Alternate Names:
    D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG PE-conjugated Control

Ctrl IC105P
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG PE-conjugated Antibody

Ctrl IC1051P
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human STAT6 (Y641) PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies