Human Survivin Fluorescein-conjugated Antibody

Discontinued Product

IC6472F has been discontinued.
Detection of Survivin in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (1)
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Human Survivin Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Survivin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 91630
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Survivin
Met1-Asp142
Accession # O15392
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Survivin in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of Survivin in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Survivin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC6472F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Survivin

Survivin is a member of the inhibitor of apoptosis (IAP) family and can inhibit apoptosis induced by a variety of factors. It is expressed in most human cancers but not in normal adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.

Entrez Gene IDs
332 (Human)
Alternate Names
API4 survivin variant 3 alpha; API4; Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; BIRC5; EPR-1; IAP4; Survivin

Product Datasheets

Citation for Human Survivin Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Indirubin-3'-monoxime, a CDK inhibitor induces growth inhibition and apoptosis-independent up-regulation of survivin in transitional cell cancer.
    Authors: Perabo FG, Frossler C, Landwehrs G, Schmidt DH, von Rucker A, Wirger A, Muller SC
    Anticancer Res., 2006;26(3):2129-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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