Detects human Synuclein-beta in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Synuclein-alpha and less than 1% cross-reactivity with rhSynuclein-gamma is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human Synuclein-beta Gly47-Glu120 Accession # Q16143
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Synuclein-beta in Human Brain. Synuclein‑ beta was detected in immersion fixed paraffin-embedded sections of human brain (globus pallidus) using Sheep Anti-Human Synuclein‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5528) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the presynaptic terminals. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Synuclein-beta by Western Blot. Western blot shows lysates of human brain cortex, Alzheimer grey matter and white matter, and human cerebellum, brain cortex, and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Human Synuclein‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Synuclein-beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Synuclein-beta is a 19 kDa, 134 amino acid (aa) cytoplasmic phosphoprotein that is a member of a family of small, highly conserved proteins, including synucleins alpha, beta, and gamma (synoretin), localized predominantly in presynaptic nerve terminals in the brain. Synuclein-beta associates with Synuclein-alpha, preventing it from aggregating. Abnormal Synuclein-alpha aggregates form filamentous inclusions (Lewy bodies) in neurodegenerative diseases including Parkinson's disease. Within the region used as an immunogen, human SNCB shares 99% aa identity with mouse and rat Synuclein-beta.