Detection of THSD1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human THSD1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5178P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
THSD1 (Thrombospondin type 1 Domain-containing protein 1), also known as TMTSP, is a 90‑100 kDa type I transmembrane protein that is reminiscent of Unc5h proteins. It is expressed on embryonic endothelial and hematopoietic stem cells, and may be involved in cell-cell interactions. Mature human THSD1 (SwissProt #: Q9NS62) contains a 389 amino acid (aa) extracellular domain (ECD) (aa 25‑413) and a 418 aa cytoplasmic region (aa 435‑852). The ECD possesses one TSP type-1 domain (aa 340‑393). There is one potential alternate start site at Met380, and two splice variants, isoform 2 of which shows a deletion of aa 341‑393 (the isoform found in NP_954872 and used here to generate this monoclonal), and isoform 3 that shows a 36 aa substitution for aa 394‑857, generating a soluble form. Over aa 25‑361 of isoform 2, human THSD1 is 76% aa identical to mouse THSD1.
Thrombospondin, Type I, Domain Containing 1
Entrez Gene IDs:
55901 (Human); 56229 (Mouse); 364630 (Rat)
thrombospondin type-1 domain-containing protein 1; thrombospondin, type I, domain 1; thrombospondin, type I, domain containing 1,4833423O18Rik; TMTSP; TMTSPMGC74971; Transmembrane molecule with thrombospondin module; UNQ3010
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