Home / TNF RII/TNFRSF1B / Human TNF RII/TNFRSF1B APC-conjugated Antibody

Human TNF RII/TNFRSF1B APC-conjugated Antibody

Catalog #: FAB226A
3 Citations Datasheet / COA / SDS
Newer Version Available: FAB226G
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Discontinued Product

FAB226A has been discontinued and is replaced by FAB2262N. FAB226S. FAB226R. FAB226T. FAB226G.

Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry.
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Citations (3)
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Human TNF RII/TNFRSF1B APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymophotactin beta  R, rhNGF R, rhOPG, recombinant mouse (rm) OX40, rhRANK, rhTAJ, rhTNF RI, or rmTNF RII.
Source
Monoclonal Mouse IgG2A Clone # 22235
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TNF RII
Extracellular domain
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TNF RII/TNFRSF1B antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TNF RII/TNFRSF1B APC-conjugated Monoclonal Antibody (Catalog # FAB226A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RII/TNFRSF1B

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a high-affinity receptor for TNF‑ alpha and TNF-beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.

Long Name
Tumor Necrosis Factor Receptor II
Entrez Gene IDs
7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)
Alternate Names
CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

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Citations for Human TNF RII/TNFRSF1B APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. TNFR2 expression is a hallmark of human memory B cells with suppressive function
    Authors: O Ticha, P Slanina, L Moos, J Stichova, M Vlkova, I Bekeredjia
    European Journal of Immunology, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Genomic analysis of mycosis fungoides and Sezary syndrome identifies recurrent alterations in TNFR2.
    Authors: Ungewickell A, Bhaduri A, Rios E, Reuter J, Lee C, Mah A, Zehnder A, Ohgami R, Kulkarni S, Armstrong R, Weng W, Gratzinger D, Tavallaee M, Rook A, Snyder M, Kim Y, Khavari P
    Nat Genet, 2015;47(9):1056-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Differential expression of TNFR1 (CD120a) and TNFR2 (CD120b) on subpopulations of human monocytes.
    Authors: Hijdra D, Vorselaars A, Grutters J, Claessen A, Rijkers G
    J Inflamm (Lond), 2012;9(1):38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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