Human Total beta-Catenin DuoSet IC ELISA

Catalog # Availability Size / Price Qty
DYC1329E
DYC1329-5
DYC1329-2
Product Details
Citations (7)
FAQs
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Human Total beta-Catenin DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total beta-Catenin in cell lysates. An immobilized capture antibody specific for beta-Catenin binds both phosphorylated and unphosphorylated beta-Catenin. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*


Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta-Catenin

beta-Catenin, also known as CTNNB1, is a cadherin-associated protein that is involved in the regulation of cell adhesion. This multifunctional protein is also implicated in the canonical Wnt signal transduction pathway, acting as a transcriptional co-activator. Nuclear beta-Catenin activates the TCF/LEF family of transcription factors, inducing the transcription of pro-mitotic genes.

Entrez Gene IDs:
1499 (Human); 12387 (Mouse); 84353 (Rat)
Alternate Names:
bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923

Citations for Human Total beta-Catenin DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Comparison of Anticancer Effects of Carbamazepine and Valproic Acid
    Authors: L Akbarzadeh, T Moini Zanj, M Sabetkasae
    Iran Red Crescent Med J, 2016;18(10):e37230.
    Species: Human
    Sample Types: Cell Lysates
  2. Incomplete epithelial-mesenchymal transition in p16-positive squamous cell carcinoma cells correlates with beta-catenin expression.
    Authors: Umbreit C, Flanjak J, Weiss C, Erben P, Aderhold C, Faber A, Stern-Straeter J, Hoermann K, Schultz J
    Anticancer Res, 2014;34(12):7061-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Pharmaceutical inhibition of glycogen synthetase kinase-3beta reduces multiple myeloma-induced bone disease in a novel murine plasmacytoma xenograft model.
    Authors: Gunn WG, Krause U, Lee N, Gregory CA
    Blood, 2011;117(5):1641-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. The Metastasis-Associated microRNA miR-516a-3p Is a Novel Therapeutic Target for Inhibiting Peritoneal Dissemination of Human Scirrhous Gastric Cancer.
    Authors: Takei Y, Takigahira M, Mihara K, Tarumi Y, Yanagihara K
    Cancer Res., 2011;71(4):1442-1453.
    Species: Human
    Sample Types: Cell Lysates
  5. Carrageenan reduces bone morphogenetic protein-4 (BMP4) and activates the Wnt/beta-catenin pathway in normal human colonocytes.
    Authors: Bhattacharyya S, Borthakur A, Dudeja PK, Tobacman JK
    Dig. Dis. Sci., 2007;52(10):2766-74.
    Species: Human
    Sample Types: Cell Lysates
  6. Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway.
    Authors: Matushansky I, Hernando E, Socci ND, Mills JE, Matos TA, Edgar MA, Singer S, Maki RG, Cordon-Cardo C
    J. Clin. Invest., 2007;117(11):3248-57.
    Species: Human
    Sample Types: Cell Lysates
  7. Quantitative in situ analysis of beta-catenin expression in breast cancer shows decreased expression is associated with poor outcome.
    Authors: Dolled-Filhart M, McCabe A, Giltnane J, Cregger M, Camp RL, Rimm DL
    Cancer Res., 2006;66(10):5487-94.
    Species: Human
    Sample Types: Cell Lysates

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