Human Total beta-Catenin DuoSet IC ELISA

  (7 citations)     
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Citations (7)
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    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Assay Length
      4 hours 40 mins (after plate preparation)
    • Sample Volume Required
      Cell lysates (100 µL)
    • Sufficient Materials
      Kits available for two, five, or fifteen 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total beta-Catenin in cell lysates. An immobilized capture antibody specific for beta-Catenin binds both phosphorylated and unphosphorylated beta-Catenin. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Available in 2, 5, and 15- (96-well) plate pack sizes
    • Economical alternative to Western blot
    Kit Content
    • Capture Antibody
    • Conjugated Detection Antibody
    • Calibrated Immunoassay Standard or Control
    • Streptavidin-HRP
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Lysis Buffer*

    IC Diluent*

    Blocking Buffer*


    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: beta-Catenin
    beta-Catenin, also known as CTNNB1, is a cadherin-associated protein that is involved in the regulation of cell adhesion. This multifunctional protein is also implicated in the canonical Wnt signal transduction pathway, acting as a transcriptional co-activator. Nuclear beta-Catenin activates the TCF/LEF family of transcription factors, inducing the transcription of pro-mitotic genes.
    • Entrez Gene IDs:
      1499 (Human); 12387 (Mouse); 84353 (Rat)
    • Alternate Names:
      bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Sample Type
  1. Comparison of Anticancer Effects of Carbamazepine and Valproic Acid
    Authors: L Akbarzadeh, T Moini Zanj, M Sabetkasae
    Iran Red Crescent Med J, 2016;18(10):e37230.
    Species: Human
    Sample Type: Cell Lysates
  2. Incomplete epithelial-mesenchymal transition in p16-positive squamous cell carcinoma cells correlates with beta-catenin expression.
    Authors: Umbreit C, Flanjak J, Weiss C, Erben P, Aderhold C, Faber A, Stern-Straeter J, Hoermann K, Schultz J
    Anticancer Res, 2014;34(12):7061-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Pharmaceutical inhibition of glycogen synthetase kinase-3beta reduces multiple myeloma-induced bone disease in a novel murine plasmacytoma xenograft model.
    Authors: Gunn WG, Krause U, Lee N, Gregory CA
    Blood, 2011;117(5):1641-51.
    Species: Mouse
    Sample Type: Tissue Homogenates
  4. The Metastasis-Associated microRNA miR-516a-3p Is a Novel Therapeutic Target for Inhibiting Peritoneal Dissemination of Human Scirrhous Gastric Cancer.
    Authors: Takei Y, Takigahira M, Mihara K, Tarumi Y, Yanagihara K
    Cancer Res., 2011;71(4):1442-1453.
    Species: Human
    Sample Type: Cell Lysates
  5. Carrageenan reduces bone morphogenetic protein-4 (BMP4) and activates the Wnt/beta-catenin pathway in normal human colonocytes.
    Authors: Bhattacharyya S, Borthakur A, Dudeja PK, Tobacman JK
    Dig. Dis. Sci., 2007;52(10):2766-74.
    Species: Human
    Sample Type: Cell Lysates
  6. Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway.
    Authors: Matushansky I, Hernando E, Socci ND, Mills JE, Matos TA, Edgar MA, Singer S, Maki RG, Cordon-Cardo C
    J. Clin. Invest., 2007;117(11):3248-57.
    Species: Human
    Sample Type: Cell Lysates
  7. Quantitative in situ analysis of beta-catenin expression in breast cancer shows decreased expression is associated with poor outcome.
    Authors: Dolled-Filhart M, McCabe A, Giltnane J, Cregger M, Camp RL, Rimm DL
    Cancer Res., 2006;66(10):5487-94.
    Species: Human
    Sample Type: Cell Lysates

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