Human Total DDR1 DuoSet IC ELISA

    
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    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Assay Length
      4 hours 40 mins (after plate preparation)
    • Sample Volume Required
      Cell lysates (100 µL)
    • Sufficient Materials
      Kits available for two, five, or fifteen 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total DDR1 in cell lysates. An immobilized capture antibody specific for DDR1 binds both phosphorylated and unphosphorylated DDR1. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Available in 2, 5, and 15- (96-well) plate pack sizes
    • Economical alternative to Western blot
    Kit Content
    • Capture Antibody
    • Conjugated Detection Antibody
    • Calibrated Immunoassay Standard or Control
    • Streptavidin-HRP
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Lysis Buffer*

    IC Diluent*

    Blocking Buffer*


    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: DDR1
    DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120-140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases and serve as receptors for collagen. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers. Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region. Two additional kinase deficient splice forms are expressed in colon cancer. The discoidin-like domain mediates binding to collagens I-V. DDR1 selectively recognizes the triple helical structure of collagen. It is expressed on the cell surface as a dimer which can include different isoforms. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization. Collagen binding induces prolonged autophosphorylation, including the NPxY motif. Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment. TIMP-3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF. Overexpression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not.
    • Long Name:
      Discoidin Domain Receptor 1
    • Entrez Gene IDs:
      780 (Human)
    • Alternate Names:
      CAK; CD167 antigen-like family member A; CD167a antigen; CD167a; DDR; DDR1; discoidin domain receptor family, member 1; discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; EC 2.7.10; EC 2.7.10.1; EDDR1; ENTRK4; Epithelial discoidin domain receptor 1; HGK2; MCK10; MCK-10; NTRK4; Protein-tyrosine kinase 3A; PTK3A protein tyrosine kinase 3A; PTK3A; receptor, type 4; RTK6; TRK E; TrkE

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