Human TRA-1-81 APC-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TRA-1-81 in flow cytometry.
  • Source
    Monoclonal Mouse IgM Clone # TRA-1-81
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human embryonal carcinoma cell line 2102Ep
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 APC‑conjugated Monoclonal Antibody (Catalog # FAB8495A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-81
The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells.  TRA-1-81 is an epitope expressed on podocalyxin, also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I transmembrane glycoprotein.
  • Long Name:
    Tumor-related Antigen-1-81
  • Entrez Gene IDs:
    5420 (Human)
  • Alternate Names:
    gp200; MGC138240; PC; PCLP; PODXL; TRA181; TRA-1-81
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Mouse IgM APC-conjugated Control

Ctrl IC015A
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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