Human TRAIL R2/TNFRSF10B PerCP-conjugated Antibody

FAB6311C has been discontinued. View all TRAIL R2/TNFRSF10B products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human TRAIL R2/TNFRSF10B in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or recombinant mouse TRAIL R2/TNFRSF10B.
  • Source
    Monoclonal Mouse IgG2B Clone # 71908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
    Ile56-Glu182
    Accession # O14763
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRAIL R2/TNFRSF10B in MBA‑MB‑123 Human breast Cell Line by Flow Cytometry. MBA‑MB‑123 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B PerCP‑conjugated Monoclonal Antibody (Catalog # FAB6311C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 Ligand). In the TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

  • References:
    1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
    2. Walczak, H. et al. (1997) EMBO J. 16:5386.
    3. Golstein, P. (1997) Curr. Biol. 7:R750.
  • Long Name:
    TRAIL Receptor 2
  • Entrez Gene IDs:
    8795 (Human); 21933 (Mouse)
  • Alternate Names:
    CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9
Related Research Areas

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