Human TRIM5 Antibody

Catalog # Availability Size / Price Qty
AF3684
AF3684-SP
Product Details
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Human TRIM5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRIM5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRIM5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TRIM5
Ala2-Gln248
Accession # Q9C035
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TRIM5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM5

TRIM5 belongs to the large family of TRIM proteins, which are also named RBCC proteins. These proteins contain a RING domain, one or two B-boxes (CHC3H2 zinc finger motifs), and the coiled-coil domain. Different TRIM proteins contain C-terminals that vary in length and composition. They also have different functions and cellular localization. TRIM5 possesses a B30.2 (also known as RFP-like or PRY-SPRY) C-terminal domain and exists as a homotrimer. TRIM5 is a single protein RING finger E3 ubiquitin ligase and has been shown to have antiretroviral activities. Five splice variants that have identical amino-terminal residues 1‑248, but differ in their C-terminal regions have been reported. Human TRIM5 shares 49% amino acid sequence homology with mouse TRIM5.

Long Name
Tripartite Motif-containing 5
Entrez Gene IDs
85363 (Human)
Alternate Names
EC 6.3.2; EC 6.3.2.-; RING finger protein 88; RNF88; RNF88tripartite motif protein TRIM; TRIM5; TRIM5alpha; tripartite motif containing 5; tripartite motif protein TRIM5; tripartite motif-containing 5; tripartite motif-containing protein 5

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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