Human TSLP R Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human TSLP R in direct ELISAs and Western blots. Does not cross‑react with recombinant mouse TSLP R.
  • Source
    Monoclonal Mouse IgG1 Clone # 147036
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TSLP R
    Accession # Q9HC73
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSLP R in NS0 Mouse Cell Line Transfected with Human TSLP R by Flow Cytometry. NS0 mouse myeloma cell line transfected with human TSLP R was stained with Mouse Anti-Human TSLP R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB981G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TSLP R

TSLP R, also named Delta (1) and CRLM-2 (2) (Cytokine Receptor-Like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3‑6). The TSLP R and IL-7 R alpha are co‑expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).

  • References:
    1. Fujio, K. et al. (2000) Blood 95:2204.
    2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
    3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
    4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
    5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
    6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  • Long Name:
    Thymic Stromal Lymphopoietin Receptor
  • Entrez Gene IDs:
    64109 (Human); 57914 (Mouse); 171499 (Rat)
  • Alternate Names:
    CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP receptor; TSLPR
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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