Human TSPAN2 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human TSPAN2 in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 822509
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human TSPAN2
    Accession # O60636
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSPAN2 in NS0 Mouse Cell Line Transfected with Human TSPAN2 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human TSPAN2 and eGFP was stained with either (A) Mouse Anti-Human TSPAN2 PE‑conjugated Monoclonal Antibody (Catalog # FAB7876P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TSPAN2
Human TSPAN2 (Tetraspanin-2) is a 34-38 kDa member of the transmembrane 4 superfamily that is closely related to CD9 and CD81. It is a cell surface, 221 amino acid (aa) glycoprotein with 4 transmembrane segments and a large, second extracellular domain loop (aa 112‑188) that contains four conserved cysteines and one N-glycosylation site. Both the N- and C-termini are intracellular. In humans, TSPAN2 has been identified on tumor cells and beta -cells of the pancreatic islets, and expressed sequence tags have been found in the pregnant uterus, on T cells, and in fetal heart. It is known to complex with Integrin alpha 2 beta 1 and CD44, and facilitate cell motility. In rats, TSPAN2 is thought to be involved in oligodendrocyte differentiation and is found on oligodendrocyte progenitors and select cerebellar neurons. It is known to participate in the formation of a molecular web with rat CD9. Notably, it is likely that human and rodent TSPAN2 have markedly different expression patterns. Human TSPAN2 shares 94% and 95% aa sequence identity with mouse and rat TSPAN2, respectively.
  • Long Name:
    Tetraspanin 2
  • Entrez Gene IDs:
    10100 (Human); 70747 (Mouse); 64521 (Rat)
  • Alternate Names:
    FLJ12082; NET3; new EST tetraspan 3; tetraspan 2; Tetraspan NET-3; tetraspan TM4SF; tetraspanin 2 isoform; tetraspanin 2; tetraspanin-2; TSN2; TSPAN-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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