Human uPAR DuoSet ELISA

  (4 citations)
(2 Reviews)
    
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Citations (4)
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    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Assay Length
      4 hours 40 minutes (after plate preparation)
    • Sample Volume Required
      100 µL
    • Sufficient Materials
      For fifteen 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    Ancillary Reagent Kit Available
    DY008 , DuoSet ELISA Ancillary Reagent Kit 2 - Please Inquire
    This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human uPAR. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Economical alternative to complete kits
    Kit Content
    • Capture Antibody
    • Detection Antibody
    • Recombinant Standard
    • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Reagent Diluent*

    Blocking Buffer*

    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: uPAR
    The urokinase-type Plasminogen Activator Receptor (uPAR) is a GPI-anchored protein that binds to both the inactive and active forms of uPA. This interaction serves to localize and enhance the proteolytic activity of uPA, leading to increased conversion of Plasminogen to Plasmin. uPA also triggers uPAR-mediated signal transduction, activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can additionally regulate cell adhesion by direct binding to Vitronectin and preventing Integrins from binding to their ligands.
    • Long Name:
      Urokinase-type Plasminogen Activator Receptor
    • Entrez Gene IDs:
      5329 (Human); 18793 (Mouse)
    • Alternate Names:
      CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Sample Type
  1. Human cytomegalovirus interleukin-10 enhances matrigel invasion of MDA-MB-231 breast cancer cells
    Authors: CA Valle Oseg, JV Spencer
    Cancer Cell Int, 2017;17(0):24.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels.
    Authors: Portelli M, Siedlinski M, Stewart C, Postma D, Nieuwenhuis M, Vonk J, Nurnberg P, Altmuller J, Moffatt M, Wardlaw A, Parker S, Connolly M, Koppelman G, Sayers I
    FASEB J, 2014;28(2):923-34.
    Species: Human
    Sample Type: Serum
  3. PLAUR polymorphisms are associated with asthma, PLAUR levels, and lung function decline.
    Authors: Barton SJ, Koppelman GH, Vonk JM, Browning CA, Nolte IM, Stewart CE, Bainbridge S, Mutch S, Rose-Zerilli MJ, Postma DS, Maniatis N, Henry AP, Hall IP, Holgate ST, Tighe P, Holloway JW, Sayers I
    J. Allergy Clin. Immunol., 2009;123(6):1391-400.e17.
    Species: Human
    Sample Type: Plasma
  4. Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
    Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
    J. Immunol., 2007;179(10):7166-75.
    Species: Human
    Sample Type: Urine

FAQs

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Substrate Reagent Pack

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Stop Solution 2N Sulfuric Acid

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Quantikine ELISA Wash Buffer 1

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ELISA Plate-coating Buffer

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Reagent Diluent Concentrate 3 (5X)

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Black Polystyrene Microplates, 25 Pack

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Average Rating: 4 (Based on 2 reviews)

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 Human uPAR DuoSet ELISA DY807 Array DY807
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  Anonymous 09/26/2016
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 Human uPAR DuoSet ELISA DY807
: Human uPAR DuoSet ELISA [DY807].

Summary

Sample Tested Serum,EDTA Plasma

Other Experimental Details

Other Experimental Details We run human serum and plasma samples at an 1:10 dilution for this assay.
 Human uPAR DuoSet ELISA DY807 Array DY807
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Average
  Anonymous 06/27/2016
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 Human uPAR DuoSet ELISA DY807
: Human uPAR DuoSet ELISA [DY807].

Summary

Sample Tested Positive controls

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