Human VEGF (Bevacizumab) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9947-100
MAB9947-SP
Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF (Bevacizumab) Antibody.
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Human VEGF (Bevacizumab) Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF based on Bevacizumab therapeutic antibody.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu101
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human VEGF-A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-80 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF (Bevacizumab) Antibody. View Larger

Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF (Bevacizumab) Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human VEGF (Bevacizumab) Monoclonal Antibody (Catalog # MAB9947). The ND50 is typically 10-80 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: VEGF

Bevacizumab is a biosimilar directed against VEGF. This product can be used as the standard in pharmacokinetic (PK) assays.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Product Datasheets

FAQs

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Reconstitution Buffers

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