Human VEGF R1/Flt-1 Antibody

(23 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse VEGF R1 and less than 10% cross-reactivity with recombinant human (rh) VEGF R2 and rhVEGF R3.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1
    Ser27-His687
    Accession # NP_001153392
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human VEGF R1/Flt‑1 Fc Chimera (Catalog # 321-FL)
  • Flow Cytometry
    2.5 µg/106 cells
    HUVEC human umbilical vein endothelial cells
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human PlGF (Catalog # 264-PG) to immobilized Recombinant Human VEGF R1/Flt-1 Fc Chimera (Catalog # 321-FL) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
VEGF R1/Flt‑1 in Human Breast Cancer Tissue. VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
VEGF R1/Flt‑1 in Human Ovarian Cancer Tissue. VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

  • Long Name:
    Vascular Endothelial Growth Factor Receptor 1
  • Entrez Gene IDs:
    2321 (Human); 14254 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Ferrochelatase is a therapeutic target for ocular neovascularization
    Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis.
    Authors: Kim H, Kim K, Kim B, Cho M, Lee S
    PLoS ONE, 2015;10(4):e0124909.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration.
    Authors: Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S
    PLoS ONE, 2015;10(9):e0138587.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice.
    Authors: Szalai G, Romero R, Chaiworapongsa T, Xu Y, Wang B, Ahn H, Xu Z, Chiang P, Sundell B, Wang R, Jiang Y, Plazyo O, Olive M, Tarca A, Dong Z, Qureshi F, Papp Z, Hassan S, Hernandez-Andrade E, Than N
    PLoS ONE, 2015;10(4):e0119547.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Melanoma spheroid formation involves laminin-associated vasculogenic mimicry.
    Authors: Larson A, Lee C, Lezcano C, Zhan Q, Huang J, Fischer A, Murphy G
    Am J Pathol, 2014;184(1):71-8.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC
  6. Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
    Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
    Mol Ther, 2014;22(7):1243-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  7. Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis.
    Authors: Hou H, Cheng S, Chung K, Kuo M, Yeh C, Chang B, Lu H, Wang H, Yu C
    Autophagy, 2014;10(9):1509-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Merkel cell carcinoma expresses vasculogenic mimicry: demonstration in patients and experimental manipulation in xenografts.
    Authors: Lezcano C, Kleffel S, Lee N, Larson A, Zhan Q, DoRosario A, Wang L, Schatton T, Murphy G
    Lab Invest, 2014;94(10):1092-102.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Attenuation of VEGFR-2 expression by sFlt-1 and low oxygen in human placenta.
    Authors: Nevo, Ori, Lee, Dennis K, Caniggia, Isabella
    PLoS ONE, 2013;8(11):e81176.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  10. Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors.
    Authors: Vizio, Barbara, Biasi, Fiorella, Scirelli, Tiziana, Novarino, Anna, Prati, Adriana, Ciuffreda, Libero, Montrucchio, Giuseppe, Poli, Giuseppe, Bellone, Graziell
    J Transl Med, 2013;11(0):314.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  11. Differential expression of Vegfr-2 and its soluble form in preeclampsia.
    Authors: Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM
    PLoS ONE, 2012;7(3):e33475.
    Species: Human
    Sample Type: Plasma
    Application: WB
  12. gamma-Secretase and presenilin mediate cleavage and phosphorylation of vascular endothelial growth factor receptor-1.
    Authors: Cai J, Chen Z, Ruan Q, Han S, Liu L, Qi X, Boye SL, Hauswirth WW, Grant MB, Boulton ME
    J. Biol. Chem., 2012;286(49):42514-23.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Proximity Ligation Assay
  13. Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues.
    Authors: Barsoum IB, Renaud SJ, Graham CH
    Am. J. Pathol., 2011;178(6):2888-96.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.
    Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
    Authors: Lee HS, Myers A, Kim J
    Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro.
    Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L
    Chem. Biol., 2006;13(5):549-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  19. Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals.
    Authors: Akintoye SO, Lam T, Shi S, Brahim J, Collins MT, Robey PG
    Bone, 2006;38(6):758-68.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  20. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
    Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  21. All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells.
    Authors: Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E
    Leuk. Res., 2004;28(3):243-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  22. PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis.
    Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
    Exp. Cell Res., 2004;299(1):68-78.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  23. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
    Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
    Blood, 2003;102(2):638-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 23 Citations
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4 (Based on 1 review)

Have you used Human VEGF R1/Flt-1 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human VEGF R1/Flt-1 Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

Filter by Ratings
Very Good
(1)
All Ratings
Filter by Applications
ICC/IF
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Very Good
 ICC/IF Human Anonymous 07/08/2016
Show

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedCT26 mouse colorectal cancer cell line,Human liver cancer cell line
SpeciesHuman

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

Feedback

Customer Information