Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, rhVEGF R3, or rhVEGF R4.
Monoclonal Mouse IgG1 Clone # 49560
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1 Ser27-His687 Accession # NP_001153392
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of VEGF R1/Flt‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R1/Flt‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB321A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGF R1/Flt-1
Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.
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