Detection of Human VEGF R1/Flt‑1 Variant Flt1-14 by Western Blot.
Western blot shows human serum (preeclampsia). PVDF Membrane was probed with 2 µg/mL of Human VEGF R1/Flt‑1 Variant Flt1-14 Monoclonal Antibody (Catalog # MAB6564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VEGF R1/Flt‑1 Variant Flt1-14 at approximately 90 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 10.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R1/Flt-1
Human VEGF R1/FLT-1 is a type I transmembrane receptor for the angiogenic cytokine, VEGF. Alternate splicings of human VEGF R1 mRNA encode soluble, secreted isoforms that can function as inhibitors of VEGF. One, designated sFLT1-14 or sFLT1-e15a, encodes a 733 amino acid (aa), 95-135 kDa form that is produced by non-endothelial cells, notably by degenerative syncytiotrophoblasts within syncytial knots in the placenta. This form appears to be unique to primates and is produced in excess during preeclampsia of pregnancy. The peptide used as an immunogen is not present within either the full-length or alternate secreted forms of the protein.
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