Human VEGF R2/KDR/Flk-1 APC-conjugated Antibody

  (13 citations)
(2 Reviews)
    
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 89106
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1
    Ala20-Glu764
    Accession # P35968
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk‑1 APC‑conjugated Mono­clonal Antibody (Catalog # FAB357A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGF R2/KDR/Flk-1

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  • References:
    1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  • Long Name:
    Vascular Endothelial Growth Factor Receptor 2
  • Entrez Gene IDs:
    3791 (Human); 16542 (Mouse)
  • Alternate Names:
    CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Vascular CXCR4 expression promotes vessel sprouting and sensitivity to sorafenib treatment in hepatocellular carcinoma
    Authors: J Xu, J Liang, YM Meng, J Yan, XJ Yu, CQ Liu, L Xu, SM Zhuang, L Zheng
    Clin. Cancer Res, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Endothelial progenitor cells and plaque burden in stented coronary artery segments: an optical coherence tomography study six months after elective PCI
    Authors: S Otto, K Nitsche, C Jung, A Kryvanos, A Zhylka, K Heitkamp, JL Gutiérrez-, B Goebel, PC Schulze, HR Figulla, TC Poerner
    BMC Cardiovasc Disord, 2017;17(1):103.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia
    Authors: M Witkowska, A Majchrzak, B Cebula-Obr, E Wawrzyniak, T Robak, P Smolewski
    Tumour Biol, 2017;39(3):1010428317694.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016;11(4):e0152993.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker
    Nat. Biotechnol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Kinetics of circulating endothelial progenitor cells in patients undergoing carotid artery surgery
    Authors: Günay Kalender
    Ther Clin Risk Manag, 2016;12(0):1841-1847.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
    Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G
    PLoS ONE, 2014;9(1):e84179.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Circulating autoantibodies to endothelial progenitor cells: binding characteristics and association with risk factors for atherosclerosis.
    Authors: George J, Matucci-Cerinic M, Bar I, Shimoni S
    PLoS ONE, 2014;9(6):e97836.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Ovine carotid artery-derived cells as an optimized supportive cell layer in 2-D capillary network assays.
    Authors: Weinandy, Stefan, Babczyk, Patrick, Dreier, Agnieszk, Unger, Ronald E, Flanagan, Thomas C, Kirkpatrick, C James, Zenke, Martin, Klee, Doris, Jockenhoevel, Stefan
    PLoS ONE, 2014;9(3):e91664.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. E-selectin mediated adhesion and migration of endothelial colony forming cells is enhanced by SDF-1alpha/CXCR4.
    Authors: Sun J, Li Y, Graziani G, Filion L, Allan D
    PLoS ONE, 2013;8(4):e60890.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy.
    Authors: Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, DU L, Gao Y, Yin M, Ding M, Deng H
    Cell Res., 2012;22(1):194-207.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011;9(5):1056-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
    Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
    Thromb. Haemost., 2007;98(2):440-50.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
Expand to show all Citations

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 11  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4.5 (Based on 2 reviews)

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Read what people are saying who have used Human VEGF R2/KDR/Flk-1 APC-conjugated Antibody.

We have 2 review tested in 1 species: Human.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human VEGF R2/KDR/Flk-1 APC-conjugated Antibody FAB357A Array FAB357A
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Excellent
 Flow Human Anonymous 10/26/2017
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Image Details
Flow Cytometry Human VEGF R2/KDR/Flk-1 APC-conjugated Antibody FAB357A
Flow Cytometry: Human VEGF R2/KDR/Flk-1 APC-conjugated Antibody [FAB357A].

Summary

Application Flow Cytometry
Sample Tested HUVEC human umbilical vein endothelial cells
Species Human
Flow Cytometry Human VEGF R2/KDR/Flk‑1 APC‑conjugated Antibody FAB357A Array FAB357A
Enlarge
Very Good
 Flow Human Anonymous 10/26/2015
Show
Image Details
Flow Cytometry Human VEGF R2/KDR/Flk‑1 APC‑conjugated Antibody FAB357A
Flow Cytometry: Human VEGF R2/KDR/Flk‑1 APC‑conjugated Antibody [FAB357A].

Summary

Application Flow Cytometry
Sample Tested Cells from hematopoietic differentiation of hESC line H9
Species Human

Other Experimental Details

Other Experimental Details Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS+2.5%FBS
Dilution: 1:20

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