Human VEGF R2/KDR/Flk-1 Fluorescein-conjugated Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 89106
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1
    Ala20-Glu764
    Accession # P35968
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk‑1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB357F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGF R2/KDR/Flk-1

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  • References:
    1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  • Long Name:
    Vascular Endothelial Growth Factor Receptor 2
  • Entrez Gene IDs:
    3791 (Human); 16542 (Mouse)
  • Alternate Names:
    CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2/KDR/Flk-1; VEGFR; VEGFR2; VEGFR-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Applications
Sample Type
  1. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
    PLoS ONE, 2016;11(8):e0160153.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments.
    Authors: De Biasi S, Cerri S, Bianchini E, Gibellini L, Persiani E, Montanari G, Luppi F, Carbonelli C, Zucchi L, Bocchino M, Zamparelli A, Vancheri C, Sgalla G, Richeldi L, Cossarizza A
    BMC Med, 2015;13(0):277.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells.
    Authors: Xiong YQ, Sun HC, Zhang W
    Clin. Cancer Res., 2009;15(15):4838-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009;183(12):8176-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 9  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 4
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 2
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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