Human Vimentin PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Vimentin in Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 280618
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Vimentin
    Accession # P08670
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Vimentin in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Rat Anti-Human Vimentin PE-conjugated Monoclonal Antibody (Catalog # IC2105P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Vimentin

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

  • References:
    1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
    2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
    3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
    4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
    5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
    6. Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.
    7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
    8. Styers, M.L. et al. (2005) Traffic 6:359.
    9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
    10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
    11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
    12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
    13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
    14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
    15. Garg, A. et al. (2006) J. Immunol. 177:6192.
  • Entrez Gene IDs:
    7431 (Human); 22352 (Mouse); 81818 (Rat)
  • Alternate Names:
    FLJ36605; VIM; vimentin
Related Research Areas


  1. Would you expect FAB210 and IC210 to give different intracellular staining results and why?

    • Yes. FAB210 was developed to detect cells that are expressing cell surface TNF-alpha and the antibody is able to recognize cells that have bound TNF-alpha on their surface through thier receptor and membrane bound forms. IC210 on the other hand detects the cytoplasmic form o fthe TNF-alpha, that is cells that are actively secreting TNF-alpha intracellularly. If FAB210 is used for intracellular staining, there is a chance that antibody will report addivitve staining of cell surface TNF-alpha as well as intracellular TNF-alpha upon permeabilization.

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 13  
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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