Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -3a, -6, -7a, -7b, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed.
Monoclonal Mouse IgG1 Clone # 326711
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Wnt‑9a Pro48-Glu92 & Arg231-Phe304 Accession # O14904.2
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Wnt‑9a in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Wnt‑9a APC‑conjugated Monoclonal Antibody (Catalog # IC3157A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Wnt-9a (Wingless integration site family member 9a), formerly Wnt-14, is a 45-47 kDa member of the Wnt family of proteins, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in both embryo and adult. It is found in embryonic mesenchyme adjacent to cartilage, and in lung at the tips of developing bronchioles. In adult, it is found throughout the liver, in hepatocytes, stellate sinusoidal cells, biliary epithelium and endothelium. It also appears in colonic epithelium and in many tumor cell types. It would appear that Wnt-9a binds to Fzd-7 and -9. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.
Wingless-type MMTV Integration Site Family, Member 9A
Entrez Gene IDs:
7483 (Human); 216795 (Mouse)
MGC138165; MGC141991; Protein Wnt-14; wingless-type MMTV integration site family, member 14; wingless-type MMTV integration site family, member 9A; Wnt-14; WNT14protein Wnt-9a; Wnt9a; Wnt-9a
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