Human XL Cyt Disc Premixed Mag Luminex Perf Assay Kit

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  • Assay Type
    Magnetic bead-based multiplex assay for the Luminex® MAGPIX® platform
  • Format
    96-well microplate and antibody-coated magnetic beads*
  • Analytes Detected
    Please see table below.
  • Performance Validation
     
    Analytes

Analytes

Select analytes you would like to measure from the list below. Please note that analytes with the same bead region are not compatible in the same assay and when selected will populate a new assay after clicking the "continue" button.

** Initial Starting Dilutions

* Note: This analyte is in more than one assay. Please refer to the Technical Datasheet for information specific to this analyte.

 
Analytes

Select analytes you would like to measure from the list below.

** Initial Starting Dilutions

Select Your Analytes Below
Analyte Data Sheet Bead Region Standard
Curve
(pg/mL)
Cell
Culture
Supernates
Serum Plasma
B7-H1/PD-L1 View 12 75.1-54,740 1:2 1:2 1:2
CCL11/Eotaxin View 13 32.3-23,580 1:2 1:2 1:2
CCL19/MIP-3beta View 14 4.2-3,100 1:2 1:2 1:2
CCL2/MCP-1 View 15 4.2-3,070 1:2 1:2 1:2
CCL20/MIP-3alpha View 18 1.9-1,360 1:2 1:2 1:2
CCL3/MIP-1alpha View 20 13.9-3,380 1:2 1:2 1:2
CCL4/MIP-1beta View 21 91.1-66,420 1:2 1:2 1:2
CD40 Ligand View 25 536.2-390,860 1:2 1:2 1:2
CX3CL1/Fractalkine View 26 167.8-122,360 1:2 1:2 1:2
CXCL1/GRO alpha View 27 4.5-3,270 1:2 1:10 1:2
CXCL10/IP-10 View 28 2.4-1,770 1:2 1:2 1:2
CXCL2/GRO beta View 29 21.6-15,780 1:2 1:10 1:2
CXCL8/IL-8 View 30 1.6-1,170 1:2 1:2 1:2
EGF View 33 7.6-5,560 1:2 1:2 1:2
FGF-basic  View 77 11.3-8,210 1:2 1:2 1:2
Flt-3 Ligand View 35 24.1-17,590 1:2 1:2 1:2
G-CSF View 36 8.4-6,160 1:2 1:2 1:2
GM-CSF View 37 17.3-12,640 1:2 1:2 1:2
Granzyme B View 38 10.6-7,740 1:2 1:2 1:2
IFN-alpha View 39 5.1-3,730 1:2 1:2 1:2
IFN-beta View 42 3.6-2,620 1:2 1:2 1:2
IFN-gamma View 43 7180 1:2 1:2 1:2
IL-10 View 44 6.1-4,450 1:2 1:2 1:2
IL-12p70 View 45 9.8-7,180 1:2 1:2 1:2
IL-13 View 46 26.5-19,330 1:2 1:2 1:2
IL-15 View 47 19.0-13,880 1:2 1:2 1:2
IL-17A View 48 31.9-23,255 1:2 1:2 1:2
IL-17E View 51 1.8-1,290 1:2 1:2 1:2
IL-1alpha View 78 11.1-8,120 1:2 1:2 1:2
IL-1beta View 53 17.2-12,570 1:2 1:2 1:2
IL-1ra View 54 3.1-2,250 1:2 1:2 1:2
IL-2 View 55 8.3-6,050 1:2 1:2 1:2
IL-3 View 56 3.1-2,260 1:2 1:2 1:2
IL-33 View 57 16.8-12,270 1:2 1:2 1:2
IL-4 View 61 18.0-13,160 1:2 1:2 1:2
IL-5 View 62 6.9-5,060 1:2 1:2 1:2
IL-6 View 63 9.0-6,590 1:2 1:2 1:2
IL-7 View 64 3.3-2,440 1:2 1:2 1:2
PDGF-AA View 66 6.6-4,810 1:2 1:10 1:2
PDGF-AB/BB View 67 18.2-13,290 1:2 1:10 1:2
RANTES View 72 296.6-216,190 1:2 1:2 1:2
TGF-alpha View 73 8.8-6,400 1:2 1:2 1:2
TNF-alpha View 74 11.4-8,320 1:2 1:2 1:2
TRAIL View 75 25.4-18,540 1:2 1:2 1:2
VEGF View 76 6.6-4,820 1:2 1:2 1:2

* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).

Product Features
  • Simultaneously detect multiple analytes
  • Magnetic format allows for easier wash steps
  • User selects specific analytes of interest
  • Requires small sample size
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
Base Kit Contents
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent(s)
  • Streptavidin-PE
  • Wash Buffer Concentrate
  • Mixing Bottles
  • Round-bottom Microplate
  • Foil Plate Sealer
Bead Set Contents
  • Antibody-coated magnetic beads
  • Biotinylated detection antibody
Information for Compatible Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.

Analyte Catalog Number Sensitivity
(pg/mL)
High Standard
Value* (pg/mL)
Microparticle
Region
B7-H1/PD-L1 LUXLM156 6.28 54740 12
CCL11/Eotaxin LUXLM320 1.81 23580 13
CCL19/MIP-3beta LUXLM361 0.6 3100 14
CCL2/MCP-1 LUXLM279 0.6 3070 15
CCL20/MIP-3alpha LUXLM360 1.05 1360 18
CCL3/MIP-1alpha LUXLM270 1.58 3380 20
CCL4/MIP-1beta LUXLM271 18.36 66420 21
CD40 Ligand LUXLM617 110.16 390860 25
CX3CL1/Fractalkine LUXLM362 16.3 122360 26
CXCL1/GRO alpha LUXLM275 25.2 3270 27
CXCL10/IP-10 LUXLM266 0.16 1770 28
CXCL2/GRO beta LUXLM276 0.827 15780 29
CXCL8/IL-8 LUXLM208 0.36 1170 30
EGF LUXLM236 1.21 5560 33
FGF-basic LUXLM233 4.95 8210 77
Flt-3 Ligand LUXLM308 3.09 17590 35
G-CSF LUXLM214 0.62 6160 36
GM-CSF LUXLM215 1.56 12640 37
Granzyme B LUXLM2906 1.04 7740 38
IFN-alpha LUXLM9345 0.29 3730 39
IFN-beta LUXLM814 0.31 2620 42
IFN-gamma LUXLM285 1.37 4450 43
IL-10 LUXLM217 2.93 7180 44
IL-12p70 LUXLM219 2.39 19330 45
IL-13 LUXLM213 3.39 13880 46
IL-15 LUXLM247 0.26 23255 47
IL-17A LUXLM317 1.09 1290 48
IL-17E LUXLM1258 1.95 8120 51
IL-1alpha LUXLM200 0.41 12570 78
IL-1beta LUXLM201 0.25 2250 53
IL-1ra LUXLM280 5.98 6050 54
IL-2 LUXLM202 0.25 2260 55
IL-3 LUXLM203 2.63 12270 56
IL-33 LUXLM3625 1.92 13160 57
IL-4 LUXLM204 0.07 810 61
IL-5 LUXLM205 0.31 5060 62
IL-6 LUXLM206 0.38 6590 63
IL-7 LUXLM207 0.18 2440 64
PDGF-AA LUXLM221 0.86 4810 66
PDGF-AB/BB LUXLM9454 0.23 13290 67
RANTES LUXLM278 40.7 216190 72
TGF-alpha LUXLM239 0.93 6400 73
TNF-alpha LUXLM210 0.62 8320 74
TRAIL LUXLM375 2.32 18540 75
VEGF LUXLM293 1.17 4820 76

*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

 

Validated Samples and Recommended Starting Dilution
Analyte Cell Culture
Supernate
Serum EDTA
Plasma
Heparin
Plasma
B7-H1/PD-L1 1:2 1:2 1:2 1:2
CCL11/Eotaxin 1:2 1:2 1:2 1:2
CCL19/MIP-3beta 1:2 1:2 1:2 1:2
CCL2/MCP-1 1:2 1:2 1:2 1:2
CCL20/MIP-3 alpha 1:2 1:2 1:2 1:2
CCL3/MIP-1alpha 1:2 1:2 1:2 1:2
CCL4/MIP-1beta 1:2 1:2 1:2 1:2
CCL7 1:2 1:2 1:2 1:2
CD40 Ligand 1:2 1:2 1:2 1:2
CX3CL1/Fractalkine 1:2 1:2 1:2 1:2
CXCL1/GRO alpha 1:2 1:10 1:2 1:2
CXCL10/IP-10 1:2 1:2 1:2 1:2
CXCL2/GRO beta 1:2 1:10 1:2 1:2
CXCL8/IL-8 1:2 1:2 1:2 1:2
EGF 1:2 1:2 1:2 1:2
FGF-basic 1:2 1:2 1:2 1:2
Flt-3 ligand 1:2 1:2 1:2 1:2
G-CSF 1:2 1:2 1:2 1:2
GM-CSF 1:2 1:2 1:2 1:2
Granzyme B 1:2 1:2 1:2 1:2
IFN-alpha 1:2 1:2 1:2 1:2
IFN-beta 1:2 1:2 1:2 1:2
IFN-gamma 1:2 1:2 1:2 1:2
IL-10 1:2 1:2 1:2 1:2
IL-12 p70 1:2 1:2 1:2 1:2
IL-13 1:2 1:2 1:2 1:2
IL-15 1:2 1:2 1:2 1:2
IL-17A 1:2 1:2 1:2 1:2
IL-17E/IL-25 1:2 1:2 1:2 1:2
IL-1alpha 1:2 1:2 1:2 1:2
IL-1beta 1:2 1:2 1:2 1:2
IL-1ra 1:2 1:2 1:2 1:2
IL-2 1:2 1:2 1:2 1:2
IL-3 1:2 1:2 1:2 1:2
IL-33 1:2 1:2 1:2 1:2
IL-4 1:2 1:2 1:2 1:2
IL-5 1:2 1:2 1:2 1:2
IL-6 1:2 1:2 1:2 1:2
IL-7 1:2 1:2 1:2 1:2
PDGF-AA 1:2 1:10 1:2 1:2
PDGF-AB/BB 1:2 1:10 1:2 1:2
RANTES 1:2 1:2 1:2 1:2
TGF-alpha 1:2 1:2 1:2 1:2
TNF-alpha 1:2 1:2 1:2 1:2
TRAIL 1:2 1:2 1:2 1:2
VEGF 1:2 1:2 1:2 1:2

 

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200 or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

 

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below). Please note that GRO-alpha, GRO-beta, PDGF-AA/AB and PDGF-BB cannot be mixed when using serum samples due to the dilution factor required. See below.

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Product Datasheets

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    Analyte Notes

    Analytes with overlapping bead regions or other incompatibilities will be placed in their own assay.

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