Human ZAG Antibody

Catalog # Availability Size / Price Qty
AF4764
AF4764-SP

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Detection of Human ZAG by Western Blot.
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Human ZAG Antibody Summary

Species Reactivity
Human
Specificity
Detects human ZAG in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ZAG
Gln21-Ser298
Accession # Q5XKQ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ZAG antibody by Western Blot. View Larger

Detection of Human ZAG by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ZAG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4764) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ZAG at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry ZAG antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

ZAG in Human Breast Cancer Tissue. ZAG was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ZAG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4764) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry ZAG antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

ZAG in Human Mesenchymal Stem Cells. ZAG was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Goat Anti-Human ZAG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4764) at 10 µg/mL for 3 hours at room temperature. Cells were differentiated using using StemXVivo Osteogenic/Adipogenic Base Media (Catalog # CCM007) and StemXVivo Adipogenic Supplement (100X) (Catalog # CCM011). Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZAG

ZAG (zinc-alpha 2-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.

Long Name
Zinc alpha-2-Glycoprotein
Entrez Gene IDs
563 (Human); 12007 (Mouse); 25294 (Rat)
Alternate Names
alpha-2-glycoprotein 1, zinc; alpha-2-glycoprotein 1, zinc-binding; AZGP1; ZA2G; ZAG; ZAGzn-alpha-2-GP; zinc; zinc-alpha-2-glycoprotein; zn-alpha-2-glycoprotein; ZNGP1

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