Reagents to verify the multipotency of neural progenitor cells by in vitro functional differentiation.
Neural progenitor cells (NPCs) are capable of differentiating into multiple cell types including astrocytes, neurons, and oligodendrocytes. During the isolation and expansion of neural progenitor cells, it is important to functionally evaluate multipotency by assessing their ability to differentiate into multiple neural lineages.
Confirmation of multipotency is a critical experimental step when studying NPCs. The kit and protocol presented here provide a quick and straightforward technique to confirm the ability of your starting cell population to differentiate toward astrocyte, neuron, and oligodendrocyte lineages. Neural stem cell research studies most often require weeks of cell culture before an experimental hypothesis can be tested. The ability to verify multipotency at an early stage in the experimental design provides valuable insight that may prevent weeks of wasted effort and reagents. Moreover, confirmation of multipotency helps to ensure consistency among studies and reduce unwanted experimental variability.
Verification of Neural Progenitor Cell Multipotency. Neural progenitor cells were maintained in culture and differentiated towards neural lineages using the specialized media supplements supplied in the Human/Mouse/Rat Neural Lineage Functional Identification Kit (Catalog # SC028). Neural progenitor cell multipotency was functionally verified using an antibody panel to detect phenotype-specific markers for neural precursors, astrocytes, neurons, and oligodendrocytes (also supplied in the kit).